Supplier: Molecular Devices. iD5. 0. . i3. , Fisher Scienti fi c, cat. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 100 fmol 384-well (75 µL) 0. . Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. . Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Unplug the power cable from the back of the instrument. Align the two slots on the bottle holder with the two pegs on the rail. Description 1. . INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 58% for the detection module and 2. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. 800. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. D 1 1. ImageXpress Confocal HT. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. com Check our website for a current listing of worldwide distributors. . The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . With optimized reagents, option to operate the. 3. Published Dec 16, 2020 | Updated Oct 10, 2023. pH-Xtra Glycolysis Assay - Agilent. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. gen) on a Spectramax i3X luminometer. Supported Windows Operating System: See release notes. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. ZERO BIAS - scores, article reviews, protocol conditions and more. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . However, no piece of equipment is immune to breaking down. Using optimized settings for the Transcreener FP assays presented in this application note, the SpectraMax i3x. The Results. Features. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. i3x. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Assay setup for a 384-well low-volume plate. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Learn more about its features, specifications, and applications in this user guide. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. SeeSpectraMax MiniMaxImagingCytometeronpage85. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. The SoftMax® Touch Software is installed in. 6% for the on-board optics (monochromators). Price from $9. SpectraMax® i3x. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. If no sound is heard during initialization, contact technical support. Actual shake speed is based on the microplate format. SpectraMax® Microplate Readers and SoftMax® Pro Software. o For example: Ex: 485/Em 525/Cutoff 515 4. 302 low ref light. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. . The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Restart the system. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. SpectraMax M5. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. More Product Information. infoboxeu@moldev. com. Introduction. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Introduction. to View Figures. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. china@moldev. We would like to show you a description here but the site won’t allow us. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Nucleic Acid and Protein Quantitation. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 2. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). 蛍光 発光 ELISA. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. 2. Figure 4. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. Reporter Assay. no. 001 0. Register to download your free eBook today. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Image analysis provides accurate data. . Unlimited breadth of. Table 1. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. HeLa cells were plated at 15,000 cells per well in 100 µL. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. We would like to show you a description here but the site won’t allow us. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. Controls containing all reaction. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. Supported Windows Operating System: See release notes. com. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. FlexStation® 3 Multi-Mode Microplate Reader. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. The chamber. The plate was covered and incubated for one hour at room temperature. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. 과 같은 Parameter를 검사하여 평가할 수 있습니다. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Published Dec 16, 2020 | Updated Apr 13, 2023. 12911151) 0. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. Find regional support. SpectraMax® i3x. 吸収度マイクロプレートリーダー SpectraMax® Mini. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The dynamic range of the assay is about 3. 660-665 Eskdale Road. . 5 Figures from 202 Publications Thermo Fisher Scientific. Quantiferon-TB Gold in Tube Kit. user upgradeable. 0001 0. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Recommended settings for SpectraMax iD5 and i3x readers. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 9 x 10-15. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Simplified workflow: Add cellular imaging assays in a small footprint. 1 and above:. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 0´ 104 1. Find regional support. Prime function 20 amol. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. User-upgradeable detection modules allow you to expand the capabilities of your microp. Bioz Stars score: 98/100, based on 1 PubMed citations. applications, including dual luciferase and ATP assays. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. 5 decades, with sensitive detection of cells. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Download PDF. Seller Details. The CellTiter-Glo assay from Promega uses luciferase. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. to View Figures. . Figure 1. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). SpectraMax i3x Multi-Mode Detection Platform User Guide. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SoftMax Pro User’s Manual v Contents Figures. 305 reference level saturation. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Instrument. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® i3x Multi-Mode Microplate Reader evolves with your future needs and offers an unlimited breadth of application possibilities. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 2in. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. It allows you to explore cellular . The SpectraMax i3x reader provides the. Wokingham, Berkshire RG41 5TS United Kingdom. 304 signal level saturation. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x Multi-Mode Detection Platform. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. . For wavelengths in the UV range above 220 nm,. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 305 reference level saturation. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Settings not required by a reader are indicated by ‘---’. Version 7. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. 304 signal level saturation. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 7 Figures from 1126 Publications. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 030 fmol. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. 012 fmol 0. Description 1. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. See Unpacking the SpectraMax Paradigm Instrument on page 1. . Thetypeof luciferase(Renilla. . Prepare your i3/i3x instrument for shipment. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Citation 2002). Unparalleled performance on a personalized platform. pathways and protein activation and expression in one system. 0´ 104 4. SpectraMax i3, i3x, and iD5 readers. . Spectranet customer portal. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Whether you are looking for an intuitive, easy-to-use reader for. Additionally, user-installable options allow the SpectraMax i3 System to. Plaque Reduction Neutralization Test (PRNT) SARS-CoV-2 neutralization was assessed as previously described . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax i3x and Paradigm readers require the FP-FLUO. Description 1. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Danaher Inc spectramax i3x. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Background subtraction was not necessary. Note: Additional readers with similar performance are listed. . AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 0. Examples: “Press carefully to engage,” “ Do not press down. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Example: [Setup]. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Curcumin binding assay. Fluorescent Polarization: Solid black plates, as above. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Engineered for high performance, the auto dual injector cartridge includes fail safe features. Molecular Devices are known for both the reliability and the wide uses of their products. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. 3 Figures from 612 Publications Promega. Determination of anti-inflammatory. In this product video, see how the SpectraMax M5 quickly reads samples. Videos & Webinars. Get a front row view of your cells. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. Facilities. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). . Spectramax I3x, supplied by Danaher Inc, used in various techniques. Microplate reader assays requiring infrared (IR) or red-shifted. Negative samples contained only MycoAlert Assay Buffer. Ease of use. style. The dual auto injector module. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Version 7. Open the catalog to page 1. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. It allows you to explore cellular pathways and protein activation and expression in one system. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. It allows you to explore cellular pathways and protein activation and expression in one system. Danaher Inc spectramax i3x. 303 unable to cal dark current. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Use the 3. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. 2 Parallel Line Analysis Conc log [ng/ML] RLU.